ABSTRACT
The present work was to clarify whether C. parvum oocysts have different strains in human patients by using different staining, electron microscopical and genetical techniques. A trial to induce a vaccine against Cryptosporidial infection in mice was carried out using killed autoclaved Cryptosporidial oocysts. The results obtained were satisfactory. Two genotypes of C. parvum. viz: human and zoonotic genotypes were detected and described. The killed vaccine used orally gave promising results
Subject(s)
Humans , Animals, Laboratory , Cryptosporidium parvum/ultrastructure , Genotype , Vaccines, Inactivated , Microscopy, Electron, Scanning , MiceABSTRACT
Cryptosporidium parvum and C. muris appear to be different species found in calves, with different oocysts size and distribution on the gastrointestinal tract. This work presents new images of C. parvum ultrastructure in calf intestine, mainly its development in nonmicrovillous cells and the presence of microtubular structures in the membrane enveloping the microgamonts and immature oocysts.